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Journal: Cell

Article Title: Structural Basis for Regulated Proteolysis by the α-Secretase ADAM10

doi: 10.1016/j.cell.2017.11.014

Figure Lengend Snippet: Key Resources Table

Article Snippet: All software used for structure determination (XDS, Phenix, Pymol and Coot) is accessible from the Structural Biology Grid (SBGrid) Consortium at Harvard Medical School. table ft1 table-wrap mode="anchored" t5 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-ADAM10 EMD Millipore ab19026 RRIB:AB_2242320 anti-HAtag Abcam ab9110 RRIB:AB_307019 anti-GAPDH Cell Signaling 2118S RRIB:AB_561053 anti-ADAM10 (11G2) Arduise et al., 2008 anti-ADAM10 (8C7) Atapattu et. al., 2016 Chemicals, Peptides, and Recombinant Proteins In-Fusion HD Cloning Plus Clontech 638911 ESF 921 Insect Cell Culture Medium, Protein-Free Expression Systems 96001 Lipofectamine 2000 Invitrogen 11668019 100% PEG 400 Hampton Research HR2603 50%w/v PEG 8000 Hampton Research HR2535 3.5M Ammonium Sulfate Hampton Research HR2541 Mca-PLAQAV-Dpa-RSSSR-NH2 R&D Systems ES0003 Batimastat (BB94) Sigma-Aldrich 19440 Compound E EMD Millipore 565790 Critical Commercial Assays Mouse IgG1 Fab and F(ab′)2 Preparation Kit Pierce 44980 TOPO™ TA Cloning™ Kit ThermoFisher 450030 Dual-Luciferase Reporter Assay System Promega E1960 BestBac 1.0 Baculovirus Cotransfection Kit Expression Systems 91100 Deposited Data Human ADAM10 structure coordinates This paper PDB ID: 6BE6 Human ADAM10-11G2 Fab structure coordinates This paper PDB ID: 6BDZ Experimental Models: Cell Lines Spodoptera frugiperda ovarian tissue (SF9) Expression Systems 94001S RRIB:CVCL_0549 U2OS ATCC HTB-96 RRIB:CVCL_0042 U2OS ADAM10 −/− This paper MS-5 Andrawes et al., 2013 MS-5 DLL4 Andrawes et al., 2013 4E11 anti-FLAG M1 ATCC HB-9259 RRIB:CVCL_J730 Recombinant DNA pVL1393 Expression Systems 91030 pVL1393 humanADAM10 3NQ Histag This paper pcDNA3.1 (+) hygro Invitrogen V87020 pRK5M-humanADAM10 Addgene 31717 pRK5M-humanADAM10 E384A This paper pRK5M-humanADAM10 ΔMP This paper pRK5M-humanADAM10 ΔEC This paper pCAX FLAG-APP Addgene 30154 pCAX FLAG-APP-HA This paper pSpCas9(BB)-2A-GFP (PX458) Addgene 48138 pSpCas9(BB) adam10exon1-2A-GFP This paper pRL Renilla Luciferase Promega E2231 Gal4- firefly Luciferase Aster et al., 2000 Notch1-gal4 Andrawes et al., 2013 pcDNA3-ΔProMP-humanADAM17-HAtag Addgene 65221 Software and Algorithms GraphPad Prism v5.0 N/A https://www.graphpad.com/scientific-software/prism/ SBGrid Consortium Morin et al., 2013 http://sbgrid.org/software/ XDS Kabsch, 2010 http://sbgrid.org/software/ Phaser McCoy et al., 2007 http://sbgrid.org/software/ Phenix.refine Afonine et al., 2012 http://sbgrid.org/software/ Coot Emsley and Cowtan, 2004 http://sbgrid.org/software/ Pymol Schrödinger Team, 2010 http://sbgrid.org/software/ Open in a separate window Key Resources Table

Techniques: Recombinant, Clone Assay, Cell Culture, Expressing, TA Cloning, Reporter Assay, Cotransfection, Luciferase, Software